THE EFFECTIVENESS OF ANCHOVY INTAKE ON EPITHELIAL SOCKET THICKNESS POST EXTRACTION

Tecky Indriana  -  Jember University, Indonesia
Agus Sumono*  -  Fakultas Kedokteran Gigi, Universitas Jember, Jember, Indonesia, Indonesia
Kunti Sholihah  -  Fakultas Kedokteran Gigi, Universitas Jember, Jember, Indonesia, Indonesia

(*) Corresponding Author
DOI: 10.30659/odj.9.1.40-45

Background: Epithelial formation or re-epithelialization is one of the parameters in the wound closure. Nutrition has become a major factors in the successful outcome during this process. Anchovy (Stolephorus sp.) contains several proteins, vitamins, and minerals that can act as supplements to support wound closure. This research proposed to understand the effect of anchovy intake toward epithelial thickness of the rats post tooth extraction.
Method: This research was an experimental laboratory with a post-test-only control group design using 24 rats as a sample. Rats were divided into 2 groups, the control and the treatment group with 12 rats each. The mandibular left first molar of the samples was extracted, then given with aquadest (the control) and anchovy powder (the treatment) during 3, 5, and 7 days. All rats were decapitated after 24 hours from the last treatment, followed by tissue processing and staining with Hematoxylin-Eosin (HE) for examination. A microscope that connects with optilab on a magnification of 100x was used to measure the epithelial thickness on the thickest and thinnest part of the epithelial. The data were analysed with One-way ANOVA test and LSD test.
Result: The results showed that the epithelial thickness of the treatment group was significantly increased compared to the control group (p<0.05).
Conclusion: This study concludes that the anchovy (Stolephorus sp.) intake affects increasing epithelial socket thickness of the rats post tooth extraction.

Keywords: Re-epithelialization; Wound healing; Anchovy

  1. Miloro M, Ghali G E, Larsen P, Waite P.Petersons Principles of Oral and Maxillofacial Surgery. 3th ed. London: PMPH-USA, Ltd;2012.
  2. Manor Y, Mardinger O, Zaks O, Haim D, Manor A, Chaushu G. Complications Following Dental Extractions in a Mobile Dental Clinic. J Dent Oral Care Med 2015; 1(1): 101.
  3. Thorne C H, Chung K C, Gosain A K, Gurtner G C, Mehrara B J, Rubin J P, Spear S L. Grabb and Smith's plastic surgery. 7th ed.Philadelphia: Elseviers; 2013.
  4. Black, J. M. Medical Surgical Nursing. Philadelphia: Saunders Company; 2019.
  5. Pastar I, Stojadinovic O, Yin N C, Ramirez H, Nusbaum A G, Sawaya A, et al. Epithelization in Wound Healing: A Comprehensive Review. Advances in Wound Care 2014, 3(7): 445-464.
  6. Eroschenko, V.P. Atlas Histologi diFiore dengan Korelasi Fungsional. 12th ed. Philadelphia : Elseveir; 2015.
  7. Septiana D A, Sa’diyah J S, Farih N N, Ningsih J R. Pengaruh gel ekstrak daun binahong (Anredera cordifolia) konsentrasi 5% terhadap re-epitelisasi luka pasca pencabutan gigi tikus putih Wistar (Rattus norvegicus). Jurnal Kedokteran Gigi Universitas Padjadjaran 2019; 31 (3):233-238.
  8. Guo S, Di Pietro L A. Factors Affecting Wound Healing. J Dent Res 2010; 89(3): 219-229.
  9. Molnar J A, Underdown M J, Clark W A. Nutrition and Chronic Wounds. Advances in Wound Care 2014, 3(11) : 663-681.
  10. Kavalukas, Sandra L, Barbul A. Nutrition and Wound Healing: An Update. Plastic Reconstruction & Surgery 2011, 127 (1S.): 38S.
  11. Russell L. The Importance of Patients Nutritional Status in Wound Healing. Clinical 2011, 10(6).
  12. Departemen Kesehatan Republik Indonesia. Daftar Komposisi Bahan Makanan (DKBM). Jakarta: Direktorat Gizi Depkes RI; 2007.
  13. Kementerian Kelautan dan Perikanan Indonesia. Kelautan dan Perikanan dalam Angka. Jakarta: Kementerian Kelautan dan Perikanan Indonesia; 2018.
  14. Wild T, Rahbarnia A, Kellner M, Sobotka L, Eberlein T. Basics in Nutrition and Wound Healing. Nutrition 2010, 26(9): 862- 866.
  15. Barchitta M, Maugeri A, Favara G, Lio R M S, Evola G, Agodi A, et. al. Nutrition and wound healing: An overview focusing on the beneficial effects of curcumin. International Journal of Molecular Sciences 2019, 20 : 1119.
  16. Zinder R, Cooley R, Vlad L, Molanar JA. Vitamin A and Wound Healing. Nutrition in Clinical Practice 2019, 34(6):839-849.
  17. Liestia L B, Prasetyarini S, Indriana T. The potency of anchovy (Stolephorus sp.) to increase fibroblast cell in socket after tooth extraction. Makassar Dental Journal 2020; 9(1): 44-47.
  18. Naghsh N, Razavi S M, Minaiyan M, Shahabooei M, Birang R, Behfarnia P , et al. Evaluation of the effects of two different bone resorption inhibitors on osteoclast numbers and activity: An animal study. Dental Research Journal 2016; 13(6): 500-507.
  19. Indriana T. Mekanisme Percepatan Pembentukan Woven Bone pada Daerah Tarikan Pergerakan Gigi Orthodonti dengan Pemberian Ikan Teri. MKGI 2016; 1(10): 17-20.
  20. Sa G, Xuepeng X, Tianfu W, Jincheng Y, Sangang H, Yifang Z. Histological features of oral epithelium in seven animal species: As a eference for selecting animal models. European Journal of pharmacheutical science 2015; 1(1): 10-17
  21. Larjava H. Oral Wound Healing: Cell Biology and Clinical Management. UK: Wiley-Blackwell; 2012.
  22. Arisanty I P. Manajemen Perawatan Luka : Konsep Dasar. Jakarta: EGC; 2013.
  23. Smith N. A Comparative Histological and Radiographic Study of Extraction Socket Healing in the Rat. Australian Dental Journal 1974, 250-254.
  24. Dey S, Ray A, Maiti N C, Banerjee P, Chatterjee J, Maity S P, et al. Precise Segmentation and Classification of Epithelial Rete Pegs Signature in Assessing Lower Limb Wound Healing Progression. Journal of Medical and Biological Engineering 2018, 39(11).
  25. Nanci A. Ten Cate’s Oral Histology, Development, Structure, and Function. 9th Ed. St Louis: Mosby Elsevier; 2017.
  26. Puspitawati R. Struktur Makroskopik dan Mikroskopik Jaringan Lunak Rongga Mulut. Jurnal Kedokteran Gigi Universitas Indonesia 2013, 10: 462- 467.
  27. Aryati E E, Dharmayanti A W S. Manfaat Ikan Teri Segar (Stolephorus sp.) Terhadap Pertumbuhan Tulang dan Gigi. Odonto Dental Journal 2014; 1(2): 52-56.
  28. Lopez E A, Zand N, Ojo O, Snowden M J, Kochhar T. The Effect of Amino Acids on Wound Healing: A Systematic Review and Meta-Analysis onArginine and Glutamine. Nutrients 2021, 13(8):2498.
  29. Bikle D D, Xie Z, Tu C L. Calcium regulation of keratinocyte differentiation. Expert Review of Endocrinology and Metabolism 2012; 7(4) : 461-472.
  30. Subramaniam T, Fauzi M B, Lokanathan Y, Law J X. The Role of Calcium in Wound Healing. International Journal of Molecular Sciences 2021,22: 6486.
  31. Polcz M E, Barbul A. The Role of Vitamin A in Wound Healing. NCP 2019; 34 (5): 695-700.

Lisensi Creative Commons
This work is licensed under a Lisensi Creative Commons Atribusi-BerbagiSerupa 4.0 Internasional.
Contact us: Odonto Dental Journal: Jl. Raya Kaligawe Km.4, PO BOX 1054/SM Semarang, Central Java, Indonesia, 50112. Email: odontodentaljournal@unissula.ac.id
apps