Bacteria inhibition of platelet concentrates with and without pre-centrifugation metronidazole incorporation on aggregatibacter actinomycetemcomitans

Adiguna Putra Walianto*  -  , Indonesia
Sri Pramestri Lastianny  -  , Indonesia
Hendrawati Hendrawati  -  , Indonesia

(*) Corresponding Author
DOI: 10.30659/odj.9.2.183-190

Background: Local administration of antibiotics was one of many approaches for the management of localized periodontal infection with the advantages of smaller total dosage delivered into the pocket, avoiding systemic antibiotic side effects, and increasing the exposure of target microorganism to higher concentration. Incorporating antibiotics into platelet concentrates can be considered for local antibiotic administration.
Method: The sample in this study used Aggregatibacter actinomycetemcomitans which was divided into 4 partitions on each plate. Each plate was applied with Platelets Rich Fibrin (PRF) and Injectable Platelet rich fibrin (I-PRF) incorporated with metronidazole pre-centrifugation and two negative controls (PRF and I-PRF without metronidazole). The observation was performed after 1 day and 7 days of incubation by measuring inhibition zone diameter using vernier caliper. The data were analyzed using two-way ANOVA followed by post-hoc LSD.
Result: I-PRF with metronidazole had the highest bacteria inhibition compared with other groups (p<0,05) on incubation day 1 and 7; I-PRF and PRF with metronidazole showed higher bacteria inhibition compared with I-PRF and PRF without metronidazole (p<0,05).
Conclusion: Aggregatibacter actinomycetemcomitans inhibition of PRF and I-PRF incorporated with metronidazole was greater compared to PRF and I-PRF without incorporating metronidazole, and A. actinomycetemcomitans inhibition of I-PRF incorporated with metronidazole was greater than PRF incorporated with metronidazole.

Keywords: platelet rich fibrin; injectable platelet rich fibrin; antibiotic; pre-centrifugation metronidazole incorporation; inhibition

  1. Nazir MA. Prevalence of periodontal disease, its association with systemic diseases and prevention. Int J Health Sci (Qassim), 2017;11(2): 72-80
  2. da Silva-Boghossian CM, do Souto RM, Luiz RR, Colombo APV. Association of red complex, A. actinomycetemcomitans and non-oral bacteria with periodontal disease. Arch Oral Biol, 2011;56(9): 899-906
  3. Tonetti MS, Greenwell H, Kornman KS. Staging and grading of periodontitis: Framework and proposal of a new classification and case definition. J Periodontol, 2018;89(Suppl 1): S159-S172
  4. Ã…berg CH, Kelk P, Johansson A. Aggregatibacter actinomycetemcomitans: Virulence of its leukotoxin and association with aggressive periodontitis. Virulence, 2015;6(3): 188-195
  5. Graziani F, Karapetsa D, Alonso B, Herrera D. Nonsurgical and surgical treatment of periodontitis: how many options for one disease?. Periodontol 2000, 2017;75(1): 152-188
  6. Marenzi G, Qorri ME, Sammartino P, Rusciano F, Gasparro R. Platelet Concentrates in Oral Surgery: Indications and Limits. A Literature Review. Curr Dent, 2019;1(1): 12-22
  7. Kour P, Pudakalkatti PS, Vas AM, Das S, Padmanabhan S. Comparative Evaluation of Antimicrobial Efficacy of Platelet-rich Plasma, Platelet-rich Fibrin, and Injectable Platelet-rich Fibrin on the Standard Strains of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. Contemp Clin Dent, 2018;9(Suppl 2): S325-S330
  8. Varshney S, Dwivedi A, Pandey V. Antimicrobial effects of various platelet rich concentrates-vibes from in-vitro studies-a systematic review. J Oral Biol Craniofac Res, 2019;9(4): 299-305
  9. Choukroun J, Ghanaati S. Reduction of relative centrifugation force within injectable platelet-rich-fibrin (PRF) concentrates advances patients’ own inflammatory cells, platelets and growth factors: the first introduction to the low speed centrifugation concept. Eur J Trauma Emerg Surg, 2018;44(1): 87-95
  10. Fujioka-Kobayashi M, Miron RJ, Hernandez M, Kandalam U, Zhang Y, Choukroun J. Optimized Platelet-Rich Fibrin With the Low-Speed Concept: Growth Factor Release, Biocompability, and Cellular Response. J Periodontol, 2017;88(1): 112-121
  11. Miron RJ, Zhang Y. Autologous liquid platelet rich fibrin: A novel drug delivery system. Acta Biomater, 2018;75(2018): 35-51
  12. Karde PA, Sethi KS, Mahale SA, Khedkar SU, Patil AG, Joshi C.P. Comparative evaluation of platelet count and antimicrobial efficacy of injectable platelet-rich fibrin with other platelet concentrates: An in vitro study. J Indian Soc Periodontol, 2017;21(2): 97-101
  13. Klokkevold PR, 2019, Treatment of Aggressive and Atypical Forms of Periodontitis, In Newman MG, Takei HH, Klokkevold PR, Carranza FA, (Ed), Newman and Carranza’s Clinical Periodontology, 13th Edition, Elsevier, Philadelphia, 480-1, 486
  14. PejÄić A, Kesić L, Obradović R, Mirković D. Antibiotics in the Management of Periodontal Disease, Acta Facultatis Medicae Naissensis, 2010;27(2): 85-92
  15. Kapoor A, Malhotra R, Grover V, Grover D. Systemic antibiotic therapy in periodontics. Dent Res J (Isfahan), 2012;9(5): 505-515
  16. Ciancio SG, Mariotti AJ, 2019, Systemic Anti-infective Therapy for Periodontal Diseases, In Newman MG, Takei HH, Klokkevold PR, Carranza FA, (Ed), Newman and Carranza’s Clinical Periodontology, 13th Edition, Elsevier, Philadelphia, 556, 559
  17. Setiawan A, Lastianny SP, Herawati D. Efektivitas Aplikasi Madu Murni Terhadap Penyembuhan Jaringan Periodontal pada Perawatan Periodontitis Penderita Hipertensi. J Ked Gi, 2013;4(4): 228-235
  18. Polak D, Clemer-Shamai N, Shapira L. Incorporating antibiotics into platelet-rich fibrin: A novel antibiotics slow-release biological device. J Clin Periodontol, 2019;46(2): 241-247
  19. Febi QN, 2020, Uji Antibakteri dan Viskositas Gel Flavonoid Daun Tembakau Kasturi terhadap Pertumbuhan Bakteri Aggregatibacter actinomycetemcomitans, Skripsi, Universitas Jember, Jember
  20. Maghsoudi O, Ranjbar R, Mirjalili SH, Fasihi-Ramandi M. Inhibitory Activities of Platelet-Rich and Platelet-Poor Plasma on the Growth of Pathogenic Bacteria. Iran J Pathol, 2017;12(1): 79-87
  21. Bachtiar SY, Thahjaningsih W, Sianita N. Pengaruh Ekstrak Alga Cokelat (Sargassum sp.) terhadap Pertumbuhan Bakteri Escherichia coli. JMCS, 2012;1(1): 53-60
  22. Husnani, Suhaimi, Puspasari H, Sari Y. Uji Daya Hambat Kestrak Kental Daun Kratom (Mitragyna speciosa Korth) terhadap Bakteri Staphylococcus aureus sebagai Penyebab Jerawat. Medical Sains, 2020;4(2): 95-100
  23. Mozartha M, Silvia P, Sujatmiko B. Perbandingan Aktivitas Antibakteri Ekstrak Curcuma zedoaria dan Bahan Irigasi Natrium Hipoklorit 2.5% terhadap Enterococcus faecalis. JMKG, 2019;8(1): 22-29
  24. Badade PS, Mahale SA, Panjwani AA, Vaidya, PD, Warang AD. Antimicrobial effect of platelet-rich plasma and platelet-rich fibrin. Indian J Dent Res, 2016;27(3): 300-304
  25. Joshi CP, Patil AG, Karde PA, Khedkar SU, Arunkum S. Autologous Platelet Rich Fibrin as a potential antiperiopathogenic agent: An in-vitro study. IP Int J Periodontol Implantol, 2016;1(2): 50-54
  26. Yeaman MR. The role of platelets in antimicrobial host defense. Clin Infect Dis, 1997;25(5): 951-958
  27. Ceruleos AH, Romero-Quezada LC, Ledezma JCR, Contreras LL. Therapeutic uses of metronidazole and its side effects: an update. Eur Rev Med Pharmacol Sci, 2019;23(1): 397-401
  28. Jafarzadeh E, Pourmokhtar M, Tavili S. In Vitro Evaluation of the Anti-bacterial Effect of Human Platelet Concentrate. IJBC, 2016;8(1): 5-8
  29. Rafiee A, Memarpour M, Taghvamanesh S, Karami F, Karami S, Morowvat MH. Drug Delivery Assessment of a Novel Triple Antibiotic-Eluting Injectable Platelet-Rich Fibrin Scaffold: An In Vitro Study, Curr Pharm Biotechnol. 2021;22(3): 380-388
  30. Kamocki K, Nör JE, Bottino MC. Dental pulp stem cell responses to novel antibioric-containing scaffolds for regenerative endodontics. Int Endod J, 2015;48(12): 1147-1156
  31. Egle K, Salma I, Dubnika A. From Blood to Regenerative Tissue: How Autologous Platelet-Rich Fibrin Can Be Combined with Oher Materials to Ensure Controlled Drug and Growth Factor Release. Int J Mol Sci, 2021;22(21): 11553(1-18)
  32. Ghanaati S, Booms P, Orlowska A, Kubesch A, Lorenz J, Rutkowski J, Landes C, Sader R, Kirkpatrick C, Choukroun J. Advanced platelet-rich fibrin: a new concept for cell-based tissue engineering by means of inflammatory cells. J Oral Implantol, 2014;40(6): 679-689
  33. Ravi S, Santhanakrishnan M. Mechanical, chemical, structural analysis and comparative release of PDGF-AA from L-PRF, A-PRF and T-PRF – an in vitro study. Biomater Res, 2020;24: 16(1-10)

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