Cd8+ expression in oral potentially malignant disorders associated with risk factors in selected population of east Indonesia

Irene Tania Bijaya  -  Universitas Trisakti, Indonesia
Firstine Kelsi Hartanto*    -  Universitas Trisakti, Indonesia
Rahmi Amtha  -  Universitas Trisakti, Indonesia
Indrayadi Gunardi  -  Universitas Trisakti, Indonesia

(*) Corresponding Author
DOI: 10.30659/odj.10.1.52-60
Background: Oral potentially malignant disorder (OPMD) is a lesion with a high potential to turn into oral squamous cell carcinoma (OSCC). The discovery of OPMD lesions precedes most cases of OSCC. Sensitive and specific biomarkers can help in the early detection of high-risk patients, one of which is CD8+, part of the cytotoxic T lymphocytes, which have a role in eliminating cancer cells. Several studies use infiltration density and CD8+ T cells' activity as biomarkers in malignancy. This study aims to observe the expression of CD8+ in OPMD lesions and correlate with risk factors
Method: Twelve paraffin blocks samples consist of OPMD lesions. One oral lichen planus (OLP) as the positive control. Samples were stained using HE to observe the degree of dysplasia and immunohistochemistry to observe the expression of CD8+ T cells. Observation of results using a microscope with 100x magnification to select the field of view and 400x magnification to count the number of positive CD8+ T cells with a cut-off point of 500 cells from a total of 5 fields of view and determined by weak intensity (<500 cells) and strong intensity (≥ 500 cells).
Result: 61,5% of samples showed weak intensity CD8+ T cell, and 38.5% showed strong intensity. There is a significant correlation between risk factors and CD8+ expression (p=0.005).
Conclusion: Expression of CD8+ T cells with strong intensity was found in OPMD lesions with moderate to severe dysplasia. Patients with risk factors showed CD8+ T-cell expression at a weaker intensity than those without risk factors.

Keywords: OPMD; dysplasia,CD8+ T cells; biomarkers; immunohistochemistry

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