EFFECTIVENESS OF ROBUSTA COFFEE BEAN EXTRACT GEL (Coffea canephora) ON THE THICKNESS OF COLLAGEN FIBERS AFTER WISTAR RAT TOOTH EXTRACTION

Desi Sandra Sari, Siti Aisyah, Dessy Rachmawati, Rina Sutjiati, Budi Yuwono

Abstract


Background: Tooth extraction causes injury to the alveolar bone and oral mucosa which is followed by the body ’s natural response through wound healing. One of the important parameters and indicators of wound healing is collagen density. Collagen is synthesised by fibroblasts in the proliferative phase, which was formed from day 3 and reached its peak on day 7. Robusta coffee beans have active compounds in the form of anti-inflammatory and antioxidants that are effective in accelerating wound healing. Objective: To determine the effectiveness of robusta coffee bean extract gel (Coffea canephora) at concentrations of 12,5%, 25%, and 50% in increasing the thickness of collagen fibres in socket wound healing after tooth extraction in Wistar rats. Method: This type of research is laboratory experimental. The samples used were 40 male Wistar rats divided into 5 groups: negative control, positive control, 12.5% robusta coffee bean extract gel treatment, 25% robusta coffee bean extract gel treatment, and 50% robusta coffee bean extract gel treatment. The sample group was decapitated on the 3rd and 7th day after tooth extraction. Socket tissues were taken to make histological preparations stained with trichrome mallory and then measured using Adobe Photoshop CS 6.0 software. Results: The average thickness of collagen fibres in the Robusta coffee bean extract gel treatment group was higher than that in the control group (p<0,05).. The 50% Robusta coffee extract gel concentration group was more effective in increasing the number of collagen fibres than the 12.5% and 25% concentration groups.. Conclusion: Robusta coffee bean extract gel could increase the thickness of collagen in wound healing after tooth extraction.

Keywords


collagen fibers, robusta coffee bean extract gel, tooth extraction

Full Text:

PDF

References


Ministry of Health. National Riskesdas Report 2018. Jakarta: Health Research and Development Agency of the Indonesian Ministry of Health; 2018.

Yang S, Li Y, Liu C, et al. Pathogenesis and Treatment of Wound Healing in Patients with Diabetes after Tooth Extraction. Endocrinol Front (Lausanne) 2022;13; DOI: 10.3389/FENDO.2022.949535.

Ismardianita E, Widyawati, Elianora D, et al. The effectiveness methanol extract clausena excavate on number of fibroblast and density of collagen fibers after tooth extraction. Journal of Dentomaxillofacial Science 2019;4(3):170–175; DOI: 10.15562/jdmfs.v4i3.996.

Anonymous. Prevalence of Types, Frequency and Risk Factors for Complications after Exodontia. Pakistan Journal of Medicine and Dentistry 2021; DOI: 10.36283/PJMD10-1/008.

Case S, Kuin P, Banjarmasin R, et al. DIFFERENCES IN THE INCIDENCE OF DRY SOCKET IN HORMONAL CONTRACEPTIVE USERS AND THOSE WHO DO NOT USE HORMONAL CONTRACEPTIVES. 2016.

Wulandari ER, Handajani J, Rosanto YB. Effectiveness of Kirinyuh (Chromolaena Odorata) Extract on Increasing of Collagen Fibers after Tooth Extraction. 2020.

Rahmadani N, Aris Wahyukundari M, Harmono H, et al. Effectiveness of Robusta Coffee Bean Extract Gel (Coffea Canephora) on Increasing the Number of Fibroblasts on Post Gingivectomy Wound Healing. n.d.

Yanuar Ichrom Nahzi M, Dwi Raharja S. EFFECT OF MANGOSTEEN PEEL EXTRACT (GARCINIA MANGOSTANA L.) AGAINST NEUTROPHIL COUNT IN INFLAMMATORY PULP In vivo study on male Wistar rats. 2016.

Soeroso Y, Winiati Bachtiar E, Boy BM, et al. The Prospect of Chitosan on The Osteogenesis of Periodontal Ligament Stem Cells. 2012.

Calvo-Guirado J, Maté-Sánchez de Val J, Ramos-Oltra M, et al. The Use of Tooth Particles as a Biomaterial in Post-Extraction Sockets. Experimental Study in Dogs. Dent J (Basel) 2018;6(2):12; DOI: 10.3390/DJ6020012.

D RAWDM, S BLMDD. Histological Comparison of Healing Following Tooth Extraction With Ridge Preservation Using Mineralized Vs. Demineralized Freeze Dried Bone Allograft. 2011;1–14; DOI: 10.1902/JOP.2011.110270.

Sukmana BI, Budhy IT, Ardani IGAW. The potentiation of Mangifera casturi bark extract on interleukin- 1β and bone morphogenic protein-2 expressions during bone remodeling after tooth extraction. Dental Journal 2017;50(1):36–42; DOI: 10.20473/j.djmkg.v50.i1.p36-42.

Sularsih S, Wahjuningsih E. Expression of bone morphogenetic protein-2 after using chitosan gel with different molecular weight on wound healing process of dental extraction. Dental Journal 2015;48(2):53; DOI: 10.20473/j.djmkg.v48.i2.p53-58.

Minamizato T, Koga T, I T, et al. Clinical application of autogenous partially demineralized dentin matrix prepared immediately after extraction for alveolar bone regeneration in implant dentistry: a pilot study. Int J Oral Maxillofac Surg 2018;47(1); DOI: 10.1016/J.IJOM.2017.02.1279.

Kresnoadi U, Rahayu RP, Djulaeha E, et al. EXPRESSION ACTIVITY OF COLLAGEN II AND OSTEOCALCYN ALVEOLAR BONE QONSEQUENT PRESERVATION POST EXTRACTION SOCKET FILLED WITH A MIXTURE OF ALOE VERA AND GRAFT 0.5%. 2014.

Shen CL, Yeh JK, Cao JJ, et al. Green tea polyphenols mitigate bone loss of female rats in a chronic inflammation-induced bone loss model. Journal of Nutritional Biochemistry 2010;21(10):968–974; DOI: 10.1016/J.Jnutbio.2009.08.002.

Lacerda SA, Matuoka RI, Macedo RM, et al. Bone quality associated with daily intake of coffee: A biochemical, radiographic and histometric study. Braz Dent J 2010;21(3):199–204.

Tajik N, Tajik M, Mack I, et al. The Potential Effects of Chlorogenic Acid, the Main Phenolic Components in Coffee, on Health: A Comprehensive Review of the Literature. Springer Berlin Heidelberg; 2017.; DOI: 10.1007/S00394-017-1379-1.

Martauli ED. ANALYSIS OF COFFEE PRODUCTION IN INDONESIA ANALYSIS OF COFFEE PRODUCTION IN INDONESIA. 2018.

Nurman S, Yulia R, Irmayanti, et al. The optimization of gel preparations using the active compounds of arabica coffee ground nanoparticles. Sci Pharm 2019;87(4); DOI: 10.3390/SCIPHARM87040032.

Machida T, Tomofuji T, Ekuni D, et al. Severe periodontitis is inversely associated with coffee consumption in the maintenance phase of periodontal treatment. Nutrients 2014;6(10):4476–4490; DOI: 10.3390/NU6104476.

Struppek J, Walther C, Bunte K, et al. The association between coffee consumption and periodontitis: a cross-sectional study of a northern German population. Clin Oral Investig 2022;26(3):2421–2427; DOI: 10.1007/S00784-021-04208-9.

Macedo RM, Brentegani LG, de Lacerda SA. Effects of coffee intake and intraperitoneal caffeine on bone repair process – A histologic and histometric study. Braz Dent J 2015;26(2):175–180; DOI: 10.1590/0103-6440201300219.

Suryadewi NPI, Utomo RB, Bramanti I. The effect of green robust coffee gel (coffea canephora) to collagen density on gingival wound healing (an in-vivo study in wistar rats). Journal of Dentomaxillofacial Science 2023;8( 1):34–38; DOI: 10.15562/jdmfs.v8i1.1284.

Bharath N, Sowmya NK, Mehta DS. Determination of antibacterial activity of green coffee bean extract on periodontogenic bacteria like Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans: An in vitrostudy. Contemp Clin Dent 2015;6(2):166–169; doi: 10.4103/0976-237X.156036.

Fatimatuzzahro N, Ermawati T, Prasetya RC, et al.

The effect of giving robusta coffee bean extract gel (Coffea canephora) on the number of osteoblasts and osteoclasts in alveolar bone of periodontitis rats

The effect of robusta coffee bean (Coffea canephora) extract gel on the number of osteoblasts and ost. Padjadjaran Journal of Dental Researchers and Students 2020;4(2):128; DOI: 10.24198/PJDRS.V4I2.28383.

Brohem CA, De Carvalho CM, Radoski CL, et al. Comparison between fibroblasts and mesenchymal stem cells derived from dermal and adipose tissue. Int J Cosmet Sci 2013; DOI: 10.1111/ICS.12064.

Sheard J. From fibroblasts to medicinal signalling cells: a paradigm shift in translational stem cell research. In-depth Focus 2018;(2):42–44.

Kurnia PA, Ardhiyanto H, Suhartini. Potential of green tea extract (Camellia sinensis) against increasing the number of socket fibroblast cells post tooth extraction in wistar rats. e-Journal of Health Library 2015;3(1):122–127.

Meng Z, Yan C, Deng Q, et al. Curcumin inhibits LPS-induced inflammation in rat vascular smooth muscle cells in vitro via ROS-relative TLR4-MAPK/NF-κB pathways. Acta Pharmacol Sin 2013;34(7):901–11; DOI: 10.1038/APS.2013.24.

Kato H, Taguchi Y, Tominaga K, et al. Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells. Arch Oral Biol 2014;59(2):167–75; DOI: 10.1016/J.ArchoralBio.2013.11.008.

Funakoshi-Tago M, Nonaka Y, Tago K, et al. Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2. Sci Rep 2020;10(1):1–17; DOI: 10.1038/S41598-020-59380-X.

Li J, Li D, Liu X, et al. Human umbilical cord mesenchymal stem cells reduce systemic inflammation and attenuate LPS-induced acute lung injury in rats. 2012;1–11.

Pierro M, Thébaud B. MSCS in Scenarios of Infection and Inflammation: Focus on Neonatal Diseases. Curr Stem Cell Rep 2016;2(2):158–167; DOI: 10.1007/S40778-016-0045-5.

Miyajima SI, Naruse K, Kobayashi Y, et al. Periodontitis-activated monocytes/macrophages cause aortic inflammation. Sci Rep 2014;4:1–9; DOI: 10.1038/SREP05171.

Diomede F, Thangavelu SR, Merciaro I, et al. Porphyromonas gingivalis lipopolysaccharide stimulation in human periodontal ligament stem cells: Role of epigenetic modifications to the inflammation. European Journal of Histochemistry 2017;61(3):231–237; DOI: 10.4081/EJH.2017.2826.


Refbacks

  • There are currently no refbacks.


Lisensi Creative Commons
This work is licensed under a Lisensi Creative Commons Atribusi-BerbagiSerupa 4.0 Internasional.
Contact us: Odonto Dental Journal: Jl. Raya Kaligawe Km.4, PO BOX 1054/SM Semarang, Central Java, Indonesia, 50112. Email: odontodentaljournal@unissula.ac.id