POTENSI KOMBINASI EKSTRAK AKAR SIDAGURI (Sida rhombifolia L.) DAN GETAH JARAK (Jatropha curcas L.) SEBAGAI BAHAN DEVITALISASI

Maria Tanumihadja*  -  Fakultas Kedokteran Gigi, Universitas Hasanuddin, Jl Perintis Kemerdekaan KM 10 Kampus UNHAS Tamalanrea, Makassar – Sulawesi Selatan, Indonesia
Indrya Kirana Mattulada  -  Fakultas Kedokteran Gigi, Universitas Hasanuddin, Jl Perintis Kemerdekaan KM 10 Kampus UNHAS Tamalanrea, Makassar – Sulawesi Selatan, Indonesia
Nurhayaty Natsir  -  Fakultas Kedokteran Gigi, Universitas Hasanuddin, Jl Perintis Kemerdekaan KM 10 Kampus UNHAS Tamalanrea, Makassar – Sulawesi Selatan, Indonesia
Lukman Muslimin  -  Sekolah Tinggi Ilmu Farmasi Makassar, Jl Perintis Kemerdekaan KM 13,7 Daya, Makassar – Sulawesi Selatan, Indonesia

(*) Corresponding Author
DOI: 10.30659/odj.6.0.14-20

Background: Arsenic paste was used to devitalize dental pulp in some problems before surgical, but it has been proven to have many hazardous effects. So, other alternative drugs are needed that are equally effective but safer. Jatropha (Jatropha curcas L.) can cause lysis blood vessels in the pulp, whereas the sidaguri (Sidarhombifolia L.) are effective in relieving inflammation. The purpose of this study was to determine the devitalization effect of jatropha and sidaguri on rabbit animal model.
Method: Sidaguri roots were extracted using reflux method while jatropha sap was dried using lyophilization method. For easy application, the extract made into a paste (1; 1) and inserted into the cavity. After 7 days of exposure, the teeth were removed, calcified and followed to histopathological staining and COX-2 expression (IHC).
Result: Histopathological examination showed that the paste was able to cause necrosis of pulp nerve. Higher dose of the drug increased the necrosis area, but the paste is not able to reduce COX-2 expression.
Conclusion: It can be concluded that the paste has a potential to developed as devitalization agent although it is necessary to study to the reduce the inflammation process.

Keywords: Sida rhombifolia L.; Jatropha curcas L.; devitalization; antiinflammation; necrosis; cyclooxygenase

  1. Srivastava A, K K Gupta, P Tandon, and J Rajpal. Necrosis of alveolar bone secondary to endodontic treatment and its management. J Interdiscip Dentistry, 2011. 1(1): p. 41-44.
  2. Zhu Z Y. Analysis of clinical application of arsenic-free deactivating agent-Depulpin. Life Science Journal, 2013. 10(1): p. 2858-2860.
  3. Chen G and P T Sung. Gingival and localized alveolar bone necrosis related to the use of arsenic trioxide paste--two case reports. J Formos Med Assoc, 2014. 113(3): p. 187-190.
  4. Osoniyi O and F Onajobi. Coagulant and anticoagulant activities in Jatropha curcas latex. J Ethnopharmacol, 2003. 89(1): p. 101-105.
  5. Abdelgadir H A and J Van Staden. Ethnobotany, ethnopharmacology and toxicity of Jatropha curcas L. (Euphorbiaceae): A review. South African Journal of Botany, 2013. 88: p. 204-218.
  6. Tomar N S, M Sharma, and R M Agarwal. Phytochemical analysis of Jatropha curcas L. during different seasons and developmental stages and seedling growth of wheat (Triticum aestivum L.) as affected by extracts/leachates of Jatropha curcas L. Physiology and Molecular Biology of Plants, 2015. 21(1): p. 83-92.
  7. Ebuehi O A and N A Okorie. Phytochemical screening and quantification of flavonoids from leaf extract of Jatropha curcas Linn. Nig Q J Hosp Med, 2009. 19(4): p. 200-5.
  8. Abed M, T Herrmann, K Alzoubi, T Pakladok, and F Lang. Tannic acid induced suicidal erythrocyte death. Cell Physiol Biochem, 2013. 32(4): p. 1106-1116.
  9. Mattulada I K. Tampilan SEM gigi yang terpapar getah jarak (Jatropha curcas Linn). Jurnal PDGI, 2013. 62(3): p. 71-74.
  10. Mattulada I K. Pengamatan histopalogis pulpa gigi M. nemestrina yang terpapar getah Jatropha curcas. Journal of Dentomaxillofacial Science, 2008. 7(1): p. 19-25.
  11. Tanumihardja M, N Natsir, I K Mattulada, and M Lukman. Pharmacological evaluation of ethanol extract of Sida rhombifolia L. roots (Malvaceae). Journal of Chemical and Pharmaceutical Research, 2016. 8(1): p. 770-774.
  12. Tanumihardja M, N Natsir, I K Mattulada, and M Lukman. Potent anti-inflammatory effect of root of sidaguri (Sida rhombifolia L) on rat periapical lesion model. International Journal of Toxicological and Pharmacological Research, 2017. 8(6): p. 412-415.
  13. Aiyelaagbe O O, A A Hamid, E Fattorusso, O Taglialatela-Scafati, H C Schroder, and W E G Muller. Cytotoxic activity of crude extracts as well as of pure components from jatropha species, plants used extensively in African Traditional Medicine. Evidence-Based Complementary and Alternative Medicine, 2011. 2011(ID 134954): p. 1-7.
  14. Liu J, Y Yang, J Xia, X Li, Z Li, L Zhou, and M Qiu. Cytotoxic diterpenoids from Jatropha curcas cv. nigroviensrugosus CY Yang Roots. Phytochemistry, 2015. 117(Sep): p. 462-468.
  15. Chen G and P T Sung. Gingival and localized alveolar bone necrosis related to the use of arsenic trioxide paste--two case reports. J Formos Med Assoc, 2014. 113(3): p. 187-90.
  16. Zarghi A and S Arfaei. Selective COX-2 inhibitors: A review of their structure-activity relationships. Iranian journal of pharmaceutical research : IJPR, 2011. 10(4): p. 655-683.
  17. Michaud A, N Lacroix-Pepin, M Pelletier, M Daris, L Biertho, M A Fortier, and A Tchernof. Expression of genes related to prostaglandin synthesis or signaling in human subcutaneous and omental adipose tissue: Depot differences and modulation by adipogenesis. Mediators of Inflammation, 2014. 2014(ID 451620): p. 1-13.
  18. Ricciotti E and G A FitzGerald. Prostaglandins and inflammation. Arteriosclerosis, thrombosis, and vascular biology, 2011. 31(5): p. 986-1000.
  19. Kawabata A. Prostaglandin E2 and pain--an update. Biol Pharm Bull, 2011. 34(8): p. 1170-1173.
  20. Hwang S H, A T Wecksler, K Wagner, and B D Hammock. Rationally designed multitarget agents against inflammation and pain. Current medicinal chemistry, 2013. 20(13): p. 1783-1799.
  21. Tanumihadja M, I K Mattulada, N Natsir, Subehan, and L Muslimin. Pemanfaatan akar sidaguri (Sida rhombifolia L.) sebagai Obat Sakit Gigi: Hewan model peradangan periapikal akibat induksi LPS Porphyromonas gingivalis. 2017, Universitas Hasanuddin: Makassar. p. 1-27.

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