COMPARISON OF ANTIFUNGAL EFFECTS OF 50% SIWAK (SALVADORA PERSICA) ETHANOL EXTRACT WITH NYSTATIN ORAL SUSPENSION ON CANDIDA ALBICANS BIOFILM (IN VITRO STUDY)

Ayatusyifa' Maghfyrany Sumarno*  -  Faculty of Dentistry, Sultan Agung Islamic University Semarang, Indonesia
Rina Kartika Sari  -  Faculty of Dentistry, Sultan Agung Islamic University Semarang, Indonesia
Rahmawati Sri Praptiningsih  -  Faculty of Dentistry, Sultan Agung Islamic University Semarang, Indonesia

(*) Corresponding Author
DOI: 10.30659/medali.4.1.47-51

Background: Candida albicans is an opportunistic pathogen that grow and survive as a commensal. However, a number of predisposing factors can turn them into pathogens. Nystatin is an antifungal drug from the polyene group use to treat oral candidiasis. Siwak have various bioactive components that can affect oral health. Tannin, one of the chemical component of siwak, is known to have antifungal activity against Candida albicans. This study aim to compare the antifungal effect of 50% siwak and nystatin on the Candida albicans biofilm.
Method: This research method was an experimental type with a post test only control group design with a sample of 36 divided into 2 groups: 50% siwak and nystatin. Each group was incubated for 24 hours. The formation of biofilms was measured by calculating Optical Density using an ELISA-reader. Data analysis was performed using the Independent T-test.
Result: The results of the average optical density values obtained 50% siwak group was 2.64261 and nystatin group was 2.428. Independent T-test results obtained p = .138 (p> 0.05) which showed no significant difference in antifungal effects between 50% siwak and nystatin.
Conclusion: This research showed that 50% siwak extract and nystatin had an equivalent antifungal effect in reducing in vitro growth of Candida albicans biofilms.

Keywords: Candida albicans; Nystatin; Siwak

  1. Glick M. Burket’s Oral Medicine 12th Edition. Shelton, Connecticut: People’s Medical Publishing House; 2015.
  2. Pedersen Aml. Oral Infections And General Health: From Molecule To Chairside. Oral Infections And General Health: From Molecule To Chairside. Switzerland: Springer; 2015. 1–149 Hal.
  3. Mayer Fl, Wilson D, Hube B. Candida Albicans Pathogenicity Mechanisms. Virulence. 2013;4(2):119–28.
  4. Wibawa T. Candida Albicans Biofilm: Formation And Antifungal Agents Resistance. J Med Sci (Berkala Ilmu Kedokteran). 2015;44(02):1–9.
  5. Gulati M, Nobile Cj. Candida Albicans Biofilms: Development, Regulation, And Molecular Mechanisms. Microbes Infect. 2016;18(5):310–21.
  6. Haque Mm, Alsareii Sa. A Review Of The Therapeutic Effects Of Using Miswak (Salvadora Persica) On Oral Health. Saudi Med J. 2015;36(5):530–43.
  7. Maharani S, Santoso O. Pengaruh Pemberian Larutan Ekstrak Siwak (Salvadora Persica) Pada Berbagai Konsentrasi Terhadap Pertumbuhan Candida Albicans. J PDGI. 2012;61(2):61–4.
  8. Tripathi K. Essentials Of Pharmacology For Dentistry. Jaypee Brothers Medical Publishers; 2016.
  9. Dowd Fj, Johnson Bs, J. Am. Pharmacology And Therapeutics For Dentistry 7th Ed. Vol. 46. Elsevier; 2017.
  10. Apsari As, Adiguna Ms. Resistensi Antijamur Dan Strategi Untuk Mengatasi. Media Dermato-Venereologica Indones. 2013;40:89–95.
  11. Adzakiyah T, Lipoeto I, Kasuma N. Pengaruh Berkumur Dengan Larutan Ekstrak Siwak ( Salvadora Persica ) Terhadap PH Saliva Rongga Mulut. J Sains Farm Klin. 2016;2(1):74–7.
  12. Hermayanti. Uji Efek Tonikum Ekstrak Daun Ceguk ( Quisqualis Indica L .) Terhadap Hewan Uji Mencit ( Mus Musculus ). J Bionature. 2013;14:95–9.
  13. Gani Ba, Alghassani Aq, Mubarak Z, Bachtiar Ew, Bachtiar Bm. Potensi Cigarette Smoke Condensate Terhadap Peningkatan Pembentukan Biofilm Candida Albicans Isolat. J Syah Kual Dent Soc. 2017;2(1):52–62.
  14. Wijaya, C. Hanny; Rachmatillah, A. Fieki; Bachtiar Bm. Penghambatan Cajuputs Candy Terhadap Viabilitas Khamir Candida Albicans Secara In Vitro. Teknol Dan Ind Pangan Ipb. 2014;
  15. Rieuwpassa Ie, Sudjarwo I, Sumintarti S, Mudjari S, Achmad H, Rasyid F. Influence Of Acrylic Resin Denture Base Soaking Length In Siwak Extract Solution (Salvadora Persica) On The Growth Of Candida Albicans. Pesqui Bras Odontopediatria Clin Integr.2018;18(1):3–8.
  16. Munro Ca. Chitin And Glucan, The Yin And Yang Of The Fungal Cell Wall, Implications For Antifungal Drug Discovery And Therapy. Vol. 83, Advances In Applied Microbiology. Elsevier; 2013. 145–172 Hal.
  17. Gow Nar, Hube B. Importance Of The Candida Albicans Cell Wall During Commensalism And Infection. Curr Opin Microbiol. 2012;15(4):406–12.
  18. Silva S, Rodrigues Cf, Araújo D, Rodrigues Me, Henriques M. Candida Species Biofilms’ Antifungal Resistance. J Fungi. 2017;3(1).
  19. Suryaningsih A, Chumaeroh S, Benyamin B. UJI EFEKTIFITAS EKSTRAK ANGGUR MERAH (Vitis Vinivera) TERHADAP PERTUMBUHAN CANDIDA ALBICANS SECARA IN VITRO. MEDALI (Media Dent Intelekt. 2015;2:5–8.
  20. Garcia-Rubio R, de Oliveira HC, Rivera J, Trevijano-Contador N. The Fungal Cell Wall: Candida, Cryptococcus, and Aspergillus Species. Front Microbiol. 2020;10(January):1–13

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