EFFECTIVENESS OF BELUNTAS EXTRACT GEL (Pluchea indica) ON THE GROWTH OF Porphyromonas gingivalis

Irma Dewi Ratnawati*    -  , Indonesia
Lulu Sa’adah  -  Program Pendidikan Dokter Gigi Fakultas Kedokteran Gigi Universitas Islam Sultan Agung, Indonesia
Budi Suhartono  -  Departemen Ortodonsia Fakultas Kedokteran Gigi Universitas Islam Sultan Agung, Indonesia

(*) Corresponding Author
Background: Periodontal disease is common in Indonesia, especially periodontitis, with a prevalence of 74.1%. One of the main etiologies of periodontitis is Porphyromonas gingivalis which colonizes dental plaque in the stages of secondary colonization and maturation. Pluchea indica leaves contain active substances with antibacterial properties, including flavonoids, tannins, alkaloids, saponin, and atsiri oil which can be used as additional pharmacotherapeutic therapy in the treatment of periodontitis. The gel preparation has good dispersion, practical, easy to apply. Aim the study to specify the effectiveness of Pluchea indica extract gel 40%, 60% and 80% against the growth of Porphyromonas gingivalis.
Method: This research employed an in vitro experimental post-test control design. Thirty samples were split into five groups: the 40%, 60%, and 80% concentrations of the Pluchea indica leaves gel, positive control using metronidazole gel, and negative control. Samples underwent 48 hours of incubation. After being administered a Pluchea indica leaves gel, the diffusion method is used to determine the sensitivity of the test bacteria to antibacterial.
Result: The diameter of the inhibition zone formed by Pluchea indica extract gel group with a concentration of around 80% was in the category very strong. Based on the results of the Tukey post-hoc test p<0.05, there were significant differences in data results between groups.
Conclusion: At concentrations of 40%, 60, and 80%, Pluchea indica leaf extract gel demonstrated antibacterial effect against Porphyromonas gingivalis. The largest Pluchea indica extract gel inhibition zone is at an 80% concentration.

Keywords: Inhibitory zone; Gel; Pluchea indica; Porphyromonas gingivalis

  1. KEMENKES. Laporan Nasional RISKESDAS 2018, Kementrian Kesehatan RI. 2018.1-582
  2. Kotsilkov, K. and Dimitrov, R. Complex Treatment in a Patient with Severe Chronic Periodontitis (Case Report). Journal of IMAB - Annual Proceeding (Scientific Papers), 2015;21(1), pp. 687–689.
  3. Newman, Carranza K. Clinical Evaluation of the Implant Patient. Carranza’s Clinical Periodontology. 2019. 635–648 p.
  4. Quamilla N. Stres dan Kejadian Periodontitis. J syiah kuala Dent Soc. 2016;1(2):161–8.
  5. Pratiwi, R. Ratnawati, ID. Nursyaputri, F dan Indraswary, R. THE EFFECTIVENESS OF PHALERIA MACROCARPA’S LEAF NANOEMULSION GEL ON STAPHYLOCOCCUS AUREUS BIOFILM THICKNESS (IN VITRO). ODONTO Dent J. 2022;9(1):69-77.
  6. Egi, M., Soegiharto, G.S. and Evacuasiany, E. (2018). Efek Berkumur Sari Buah Tomat (Solanum lycopersicum L.) Terhadap Indeks Plak Gigi. SONDE (Sound of Dentistry). 2018; 3(2),70–84.
  7. Puspita S, Hanifatunnisa LS, Dharmayanti AWS, Mahanani ES, Saleh E. Effect of Fibroin Sponge on Alveolar Bone Remodeling Process Post Tooth Extraction. ODONTO Dent J. 2022;9(1):7–15.
  8. Septiwidyati TR, Bachtiar EW. The Role of Porphyromonas gingivalis Virulence Factors in Periodontitis Immunopathogenesis. Dentika Dent J. 2020;23(1):6–12.
  9. Braswell, L.D. and Van Dyke, T.E. The etiology and pathogenesis of periodontal disease. BAOJ Dentistr. 2018;4(2): 1–8
  10. Abdurrohman MMS, Putranto RR. Metronidazole Gel Effect on Rats With Bacteria-Induced Periodontitis. ODONTO Dent J. 2020;7(1):48.
  11. Haris M, Panickal DM. Role of Metronidazole as a Local Drug Delivery in the Treatment of Periodontitis:A Review. Int J Oral Heal Med Res. 2017;3(July):141–5.
  12. Sidiqa AN, Herryawan H. Efektifitas Gel Daun Sirih Merah (Piper crocatum) pada Perawatan Periodontitis Kronis. Kartika J Ilm Farm. 2017;5(1):1–6.
  13. Siregar IH., Supardan I, Sulistijarso N. The Effect of Paste of Sukun Leaves Extract (ArtocarpusAltilis) towards Fibroblast cell and Collagen Tissues on Periodontitis. J Ris Kesehat. 2015;4(3):786–92.
  14. Wijayanto R, Herawati D, Sudibyo. Perbedaan Efektivitas Topikal Gel Asam Hialuronat Dan Gel Metronidazol Terhadap Penyembuhan Jaringan Periodontal Setelah Kuretase Pada Periodontitis Kronis. J Ked Gi [Internet]. 2014;5(3):307–11.
  15. Slamet S, Anggun BD, Pambudi DB. Uji Stabilitas Fisik Formula Sediaan Gel Ekstrak Daun Kelor (Moringa Oleifera Lamk.). 2020;XIII(II):115–22.
  16. Yuliani I, Ardana M, Rahmawati D. Pengaruh pH terhadap Aktifitas Antibakteri Ekstrak Etanol Daun Beluntas Terhadap Bakteri Penyebab Jerawat. Proceeding Mulawarman Pharm Conf Fak Farm Univ mulawarman, samarinda. 2017;105–8.
  17. Megawati, Roosevelt A, Akhir LO. Formulasi dan Uji Stabilitas Fisik Sediaan Gel Ekstrak Kulit Buah Rambutan (Nephelium lappaceum l.) sebagai Obat Sariawan Menggunakan Variasi Konsentrasi Basis Carbopol. J Farm Sandi Karsa. 2019;5(1):5–10.
  18. Mardiana RN, Handayani N. Antibacterial Activity of the Sambiloto Leaf Extracts (Andrographis paniculata) to Bacillus cereus and Pseudomonas aeruginosa. Biofarmasi J Nat Prod Biochem. 2017;14(1):19–24.
  19. Vera A, Astri A, Hafida N, Megawati V. Pengaruh Daya Antibakteri Ekstrak Daun Stevia terhadap Streptococcus mutans (In Vitro). J Ilmu Kedokt Gigi. 2017;1(1):9–14.
  20. Alfarid ANA. Efek Terapi Ekstrak Etanol Daun Beluntas (Pluchea Indica L) pada Mencit (Mus musculus) Mastitis Subklinis Hasil Induksi Staphylococcus aureus Pembentuk Biofilm terhadap Produksi CD4 dan CD8 pada Organ Limpa. 2018;17–32.
  21. Sun X, Yang X, Xue P, Zhang Z, Ren G. Improved Antibacterial Effects of Alkali-Transformed Saponin from Quinoa Husks Against Halitosis-Belated Bacteria. BMC Complement Altern Med. 2019;19(1):1–10.
  22. Tanaka RR, Suwandi T, W AS. Effectiveness Difference of Metronidazole Gel and Tetracycline Gel Against Porphyromonas gingivalis in Vitro. 2017;
  23. Tedjasulaksana R. Metronidasol Sebagai Salah Satu Obat Pilihan untuk Periodontitis Marginalis. J Kesehat Gigi. 2016;4:19–23.

Lisensi Creative Commons
This work is licensed under a Lisensi Creative Commons Atribusi-BerbagiSerupa 4.0 Internasional.
Contact us: MEDALI Jurnal: Media Dental Intelektual : Jl. Raya Kaligawe Km.4, PO BOX 1054/SM Semarang, Central Java, Indonesia, 50112. Email: odontodentaljournal@unissula.ac.id
apps