METRONIDAZOLE GEL EFFECT ON RATS WITH BACTERIA-INDUCED PERIODONTITIS

Muhamat Muhtar S. Abdurrohman*    -  Universitas Islam Sultan Agung, Indonesia
R. Rama Putranto  -  Universitas Islam Sultan Agung, Indonesia

(*) Corresponding Author
DOI: 10.30659/odj.7.1.48-52

Background: Periodontitis is an inflammatory disease in the periodontal tissue and causes progressive tissue damage. Tissue Inhibitor of Matrix Metalloproteinases-1 (TIMP-1) is directly responsible for the pathogenesis of periodontal diseases. Topical application of the metronidazole gel as a therapy for periodontal diseases can be used for additional therapy. This study aimed to analyze the effect of Metronidazole gel on periodontitis based on TIMP-1 levels in bacteria-induced periodontitis in rats.
Methods: Twenty Sprague Dawley rats weighing 170-200 gram aged two months. Rats were divided into two groups: the control group and Metronidazole gel (Ti-es Metronidazole gel, 0.45 mg). Metronidazole gel was topically applied to the gingival sulcus. The gingival crevicular fluid sample was taken on day 3 with periopaper subjected to the Tissue Inhibitor of Matrix Metalloproteinase-1 level evaluation using the ELISA Method.
Results: Normal reference Tissue Inhibitor of Matrix Metalloproteinase 1 levels gingival crevicular fluid 0.156-10 ng/ml. Tissue Inhibitor of Matrix Metalloproteinase 1 gingiva crevicular fluid in control and metronidazole gel was 1.34 ng/ ml and 3.57 ng /ml. There was a significant difference between control and metronidazole gel group (p<0.005)
Conclusion: The Application of Metronidazole gel affects TIMP-1 levels in periodontitis.

Keywords: Metronidazole gel; Peridontitis; TIMP-1 Levels

  1. Hafez Mohd F, Al Bayaty Fouad H, Ihsan AM. Prevalence and Evaluation of Bone Loss Pattern among Patient with Aggressive Periodontitis. J Int Dent Med Res. 2017;10(3):862-867.
  2. Prasetya RC, Purwanti N, Haniastuti T. Infiltrasi Neutrofil pada Tikus dengan Periodontitis setelah Pemberian Ekstrak Etanolik Kulit Manggis. Maj Kedokt Gigi Indones. 2014;21(1):33. DOI:10.22146/majkedgiind.8520.
  3. Balli U, Cetinkaya BO, Keles GC, et al. Assessment of MMP-1, MMP-8 and TIMP-2 in experimental periodontitis treated with kaempferol. J Periodontal Implant Sci. 2016;46(2):84-95. DOI:10.5051/jpis.2016.46.2.84.
  4. Barros SP, Williams R, Offenbacher S, Morelli T. Gingival crevicular fluid as a source of biomarkers for periodontitis. Periodontol 2000. 2016;70(1):53-64. DOI:10.1111/prd.12107.
  5. Athraa Ali Mahmood, Ghasaq A. Abdul-Wahab SIA-K. Effect of Hyaluronan and Metronidazole Gels in Management of Chronic Periodontitis. J Int Oral Heal. 2020;11(June):158-163. DOI:10.4103/jioh.jioh.
  6. Ansari SA, Syed SA, Quadir F, Aslam K. Effect of 1% Metronidazole Gel as an Adjunct to Subgingival Scaling in the Treatment of Periodontitis. J Pakistan Dent Assoc. 2017;26(3):123-128. DOI:10.25301/jpda.261.123.
  7. Rajagopalan A, Thomas DJT. Effectiveness of Metronidazole as Local Drug Delivery in Periodontal Diseases – A Review. IOSR J Dent Med Sci. 2014;13(8):25-28. DOI:10.9790/0853-13842528.
  8. Minic I, Pejcic A. Pathogenesis of Aggregatibacter actinomycetemcomitans in Periodontitis. 2019;(March).
  9. Miani PK, Do Nascimento C, Sato S, Filho A V., Da Fonseca MJV, Pedrazzi V. in vivo evaluation of a metronidazole-containing gel for the adjuvant treatment of chronic periodontitis: preliminary results. Eur J Clin Microbiol Infect Dis. 2012; 31(7): 1611-1618. DOI:10.1007/s10096-011-1484-7.
  10. Reddy NR, Deepa A, Babu DSM, Chandra NS, Reddy CVS, Kumar AK. Estimation of tissue inhibitor of matrix metalloproteinase-1 levels in gingival crevicular fluid in periodontal health, disease and after treatment. J Indian Soc Periodontol. 2014;18(3):301-305. DOI:10.4103/0972-124X.134565.
  11. Matsuda S, Movila A, Suzuki M, et al. A novel method of sampling gingival crevicular fluid from a mouse model of periodontitis. 2016;438:21-25. DOI:10.1016/j.jim.2016.08.008.
  12. Taylor JJ. Protein Biomarkers of Periodontitis in Saliva. ISRN Inflamm. 2014;2014(Cvd):1-18. doi:10.1155/2014/593151.
  13. Majeed ZN, Philip K, Alabsi AM, Pushparajan S, Swaminathan D. Identification of Gingival Crevicular Fluid Sampling , Analytical Methods , and Oral Biomarkers for the Diagnosis and Monitoring of Periodontal Diseases : A Systematic Review. 2016;2016.
  14. Gulnur Erningil, Buket Han, Ali Gurkan, Afig Berdeli, Taina Tervahartiala E. Matrix Metalloproteinase ( MMP ) -8 and Tissue Inhibitor of MMP-1 ( TIMP-1 ) Gene Polymorphisms in Generalized Aggressive Periodontitis : Gingival Crevicular Fluid MMP-8 and TIMP-1 Levels and Outcome of Periodontal Therapy. J Periodontal. 2015;85(November 2013):1070-1080. DOI:10.1902/jop.2013.130365.
  15. Popat RP, Bhavsar N V, Popat PR. Gingival crevicular fluid levels of Matrix Metalloproteinase-1 ( MMP-1 ) and Tissue Inhibitor of Metalloproteinase-1 ( TIMP-1 ) in periodontal health and disease. Singapore Dent J. 2014;35:59-64. DOI:10.1016/j.sdj.2014.07.003.
  16. Prado P, Rossy B, Molina B, Prado P, Rossy B. Metalloproteinases ( MMPs ) of the extracellular matrix in Dentistry. XVIII(November 2016):19-28.
  17. Franco C, Patricia HR, Timo S, Claudia B, Marcela H. Matrix metalloproteinases as regulators of periodontal inflammation. Int J Mol Sci. 2017;18(2):1-12. DOI:10.3390/ijms18020440.
  18. 18. KJ N. Role of Matrixmetalloproteinases in Periodontal Disease - A Review. Biomed J Sci Tech Res. 2018;2(1):2099-2104. DOI:10.26717/bjstr.2017.01.000632.
  19. Botelho MA, Martins JG, Ruela RS, Queiroz DB. Nanotechnology in ligature-induced periodontitis : protective effect of a doxycycline gel with nanoparticules. 2010;18(4):335-342.
  20. Rosamma G, Kavyashree G. Effect of hyaluronan and Metronidazole gels in management of chronic periodontitis. J Int Oral Heal. 2019;11(November):158-163. DOI:10.4103/jioh.jioh.

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