Nigella Sativa Seed Extract Ointment Increasing the Amount of Healing Fibroblast and Collagen on Balb/C Mice

Sri Mastuti*  -  Wahid Hasyim University, Semarang, Central Java Indonesia, Indonesia
Djoko Tri Hadi  -  Department of Biomedical Sciences Medical Faculty, Sultan Agung Islamic University, Indonesia
Chodidjah Chodidjah  -  Department of Biomedical Sciences Medical Faculty, Sultan Agung Islamic University, Indonesia

(*) Corresponding Author
DOI: 10.30659/sainsmed.v8i1.1006

Introduction: Untreated cuts in some period of time, caused by various reasons, can be a problem. Alternative therapy used to speed up the healing of the cut is Nigella Sativa (NS).

Objective: to investigate the number of fibroblast and collagen by giving 40% and 60% NS seed extract on the cut.


Methods: post test only control group design, 48 male balb/c strain rats were divided into 2 large groups, with each cage contained 24 rats. Each group then divided to 4. Each group is subdivided into 4. Nigella sativa seed extract ointment with a concentration of 40% (NS-40) and 60% (NS-60) smeared 3x daily for 7 days (fibroblasts) and 14 days (collagen). Evaluation derived from preparations using HE staining and Masson’s Trichrome respectively.


Results: Mann Whitney analysis shows that the number of fibroblasts and collagen in the CG group, NS-40, and NS- 6 0 Higher than CN, P <0.05.
The number of fibroblasts in the NS-40 and NS-60 was not significantly different compared to the CG, as well as CN-40 with CN-60, p> 0.05. While the number of collagen in CN-60 were significantly higher than CN-40, p<0.05.


Conclusion : administration of Nigella sativa seed extract ointment with a concentration of 40% and 60% in cuts for 7-14 days increase the number of fibroblasts and collagen, resulting in a shorter healing period.

Keywords: black cumin seed extract ointment (Nigella sativa);fibroblasts;collagen;cuts.

  1. Ambiga et al., 2007. Evaluasi on of Wound Healing Activity of Flavonoids from ipomoea. Carnea Jacq. An iseng Science of life
  2. Anthony P. Morrison, Paul F, Lara W, Shon W. Lewis, Aoiffe K, Joanne G, Shophie P, Richard P. Bentall., 2004. Cognitive therapy for the prevention of psychosis in people at ultra-high risk. The British Journal of Psychiatry 185 (4) 291-297.
  3. Dharma et al., 2010. Pengaruh Suplemen Zink terhadap Pembentukan Kolagen pada Soket Gigi Marmut yang Mengalami Defisiensi Zink Pasca Pencabutan Gigi, J Ked Gi.1(3): 94-98.16
  4. Froschle M, Pluss, Peter A. Etzweiler E, Ruegg D., 2004.m Phytosteroid for Skin Care. Personal Care.558.
  5. Henry F, Chamber, MD., 2007. Miscellaneous Antimicrobial Agent : Disinfectants, Antiseptics, dan Sterilants. In: Katzung, Bertram G. Basic and Clinical Pharmacology 10th edition. New York: The Mc Graw Hill Companies, Inc. p.822.
  6. Med Market Diligence, 2009. Market intelegence for competitive advantage to the world’s leading and emerging medtech companies.http://mediligence.com. USA. cited on 6.11.2015
  7. Niluh, 2012. Pemberian salep ekstrak Jinten Hitam (Nigella sativa) terhadap peningkatan kepadatan sabut kolagen pada mukosa oral Marmut (Cavia cobaya). Oral biology dental journal vol 4 no 1 januari-juni.p 30-34
  8. Nova et al, 2013. The Effect of black cumin seeds extract toward the number of fibroblas, macrofages, and blood vessels post tooth extraction on wistar rats. Vol 2 No. 2. Oral and Maxillofacial Surgerry Journal.
  9. Parakh, P. M., 2010. Nigella sativa Linn. a comprehensive review. Indian Journal of Natural Products and Resources, I(4), 409-426.
  10. Rachman et al., 2014. Evaluation of Wound Closure Activity of Nigella Sativa, Melastoma, malabathriic, Plachea Indica and Paper Sarmentosum Extracts on Scratiches Monolayer of Human Gingival Fibroblast. MED. Kuala Lumpur, Malaysia. Evidence-Based Complementary and Alternative Medicine. Article ID 190342, 9 pages http:// dx.doi.org/10.1155/2014/190342
  11. Scultz, 2003. Wound Bed preparation: A systematic Approach Wound Management, Jurnal Wound Rep Reg 2003: 11:1-28.
  12. Suryadi, Asmarajaya, Maliawan, 2012. Proses penyembuhan dan penanganan luka dalam. http://library.unud.ac.id/index.php/eum/ article/download/4885/3671, Di akses tanggal 3 September 2015.
  13. Surkhail et al., 2011. Burns Healing Potential of Nigella Sativa Seed Oil in Rats. Vol. 2. International Journal of Pharmaceutical Science and Research. Tehran, Iran.page.: 34-40.

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ISSN: 2339-093X (Online) | 2085-1545 (Print)
DOI : 10.30659/sainsmed

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