Mulawarman University - Indonesia
EFEKTIVITAS EKSTRAK ETANOL DAUN BELUNTAS PLUCHEA INDICA (L.) LESS TERHADAP BAKTERI SALIVA SECARA IN VITRO
Annisa Fairus Syafira
Masyhudi Masyhudi
Departemen Mikrobiologi, Program Studi Kedokteran Gigi, Fakultas Kedokteran Universitas Mulawarman - Indonesia
Departemen Mikrobiologi, Program Studi Kedokteran Gigi, Fakultas Kedokteran Universitas Mulawarman
Sinar Yani
Departemen Biologi Oral, Program Studi Kedokteran Gigi, Fakultas Kedokteran Universitas Mulawarman - Indonesia
Departemen Biologi Oral, Program Studi Kedokteran Gigi, Fakultas Kedokteran Universitas Mulawarman
(*) Corresponding Author
Background: Pluchea indica (L.) Less leaf contains antibacteria substances that can inhibit the growth of bacteria that cause dental and oral diseases. Previous research shows that Pluchea indica (L.) Less leaf extract can inhibit the growth of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa bacteria. This research aimed to find out effectiveness of Pluchea indica (L.) Less leaves to decrease in the number of salivary bacteria colony.
Method: This research used a sample of saliva obtained from a person who has dental caries. The saliva had been treated with Pluchea indica (L.) Less leaves ethanol extract with concentration 2,5%, 3,5%, 4,5%, 5,5%, and 6,5%. Bacterial colonies were calculated using a colony counter and no identification was performed.
Result: The mean of bacteria colony number in Plate Count Agar media without Pluchea indica (L.) Less leaves ethanol extract is 142,67. After being given Pluchea indica (L.) Less leaves ethanol extract with concentration of 2,5%, 3,5%, and 4,5%, the mean of bacteria colony number sequentially are 15,67, 10,67, 4, and at a concentration of 5.5% and 6.5% no more bacterial colonies were found.
Conclusion: Pluchea indica (L.) Less leaves ethanol extract effective to inhibit and eliminate oral bacteria.
Keywords: cteria; Ethanol; Extraction; Pluchea indica (L.) Less; Salivary
- RI BK. Riset Kesehatan Dasar: RISKESDAS. 2017.
- Widani N, Nasution Y. Perbandingan Oral Care Menggunakan Povidone Iodine 1% dengan Chlorhexidine 0,2% Terhadap Jumlah Bakteri di Mulut Pada Pasien Penurunan Kesadaran 2015.
- Nareswari A. Perbedaan efektivitas Obat Kumur Chlorhexidine Tanpa Alkohol Dibandingkan Dengan Chlorhexidine Beralkohol Dalam Menurunkan Kuantitas Koloni Vakteri Rongga Mulut 2010.
- Swastini IGAAP. Kerusakan Gigi Merupakan Fokal Infeksi Penyebab Timbulnya Penyakit SIstemik. Jurnal Kesehatan Gigi Vol.1 No.1. 2013: 63-68.
- Brooks F, Carrol KC, Butel JS, Morse SA, Mietzner TA. Jawetz, Melnick, & Adelberg Mikrobiologi Kedokteran. Jakarta: EGC; 2005.
- Y S. A Review on the Human Oral Microflora. Research dan Reiew: Journal of Dental Sciences Volume 4. 2016.
- Lamont RJ, Jenkinson HF. Oral Microbiologi at a Glance. Singapore: Wiley Blackwell; 2010.
- Brooks GF, Carroll KC, Butel JS, Morse SA, Mietzner TA. Jawetz, Melnick, & Aldelberg Mikrobiolgi Kedokteran. Jakarta: EGC; 2014.
- Wibowo EAA. Perbandingan Kuantitas Bakteri Rongga Mulut Antara Berkumur dengan Klorheksidin dan Minyak Atsiri Bunga Cengkih (Syzygium aromaticum L.) 2010.
- Agtini MD, Sintawati, Tjahja I. Fluor dan Kesehatan Gigi. Media Litbang Kesehatan Vol. XV No. 2. 2005: 25-31.
- Kocak MM, Ozcan S, Sibel K, Topuz O, Erten H. Comparison of the Efficacy of Three Different Mouthrinse Solutions in Decreasing the Level of Streptococcus mutans in Saliva. European Journal of Dentistry. 2009: 57-61.
- Nahak MM. Ekstrak Daun Beluntas (Puchea indica L) Dapat Menghambat Pertumbuhan Bakteri Streptococcus mutans. Jurnal kesehatan Gigi Vo. 1 No.1. 2013.
- Setyowati FM. Etnofarmakologi dan Pemakaian Tanaman Obat Suku Dayak Tunjung di Kalimantan Timur. Media Litbang Kesehatan Volume XX Nomor 3. 2010.
- Hidayati RS, Napitupulu RM. Kitab Tumbuhan Obat. Jakarta: AgriFlo (Penebar Swadaya Grup); 2015.
- Febriana HM, Amintarti S, Putra AP. Pengaruh Ekstra Daun Beluntas (Pluchea indica (L) Less) Terhadap Pertumbuhan Bakteri Escherichia coli. Jurnal Wahana-Bio Volume XIII. 2015.
- (IndoBIC) IBIC. Senyawa Antimikroba dari Tanaman. 2005. Available at: http://indobic.or.id. Accessed April 28, 2018.
- Lamothe R. Plant ANtimicrobial Agents and Their Effects on Plant and Human Pathogens. Int. J. Mol Sci 10. 2009: 3400-3419.
- H AR, Cahyanto T, Sujarwo T, Lestari RI. Uji Aktivitas Antibakteri Daun Beluntas (Pluchea indica (L.) Less) Terhadap Propionibacterium Penyebab Jerawat. 2015: 141-161.
- Angelina M, Turnip M, Khotimah S. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kemangi (Ocimum sanctum L.) Terhadap Pertumbuhan Bakteri Ezcherichia coli dan Staphylococcus aureus. Jurnal Protobiont Vol.4(1). 2015: 184-189.
- Pelczar J, Chan ECS, Krieg NR. Microbiology; 1993.
- Suhartono. (2015). Skripsi Uji Efek Antibakteri Ekstrak Etanol Daun Beluntas (Pluchea indica (L) Less.) Ovenan Terhadap Escherichia coli Penyebab Diare. Samarinda.
- Wulandari V, Husain DR, Sartini, Haedar N. Pengujuan Aktivitas Antibakteri Dari Ekstrak Etanol Daun Beluntas Pluchea indica Less. Terhadap Stapphylococcus aureus dan Pseudomonan aeruginosa. 2009.
- Giordino L, Palazzi F, Zahed M, Giardino L, Palazzi F, Asgary S. Agonistic and Antagonistic Interactions between Chlorhexidine and Other Endodontic Agents: A Crtical Review. Iranian Endodontic Journal. 2014: 89-97.
