PENGARUH KONSENTRASI GEL THEOBROMINE TERHADAP KETAHANAN KEKERASAN PERMUKAAN EMAIL GIGI DESIDUI

indah lestari vidyahayati*  -  , Indonesia
Rinaldi Budi Utomo  -  Universitas Gadjah Mada
Indah Titien Soeprihati  -  , Indonesia

(*) Corresponding Author

Background: Primary tooth enamel are susceptible to damage because it has a low thickness so it requires preventing treatments to maintain. Theobromine, an alkaloid of the methylxanthine group, has been shown to improve crystallinity and resistance of enamel apatite solubility to acids. The enamel resistance to acid requires a series of test of physical properties, by measuring at microhardness. This study analyzes the effect of theobromine gel consentration on the hardness resistance of primary incisors enamel surfaces.
Method: Twenty-fve specimens were divided into 5 groups of topical application of theobromine gel with concentrations of 1%, 2%, 3%, 4%, and 5%, and then immersed in acidic solution. Results were analyzed using one-way ANOVA
Result: One-way ANOVA test showed signifcant differences in the increase and decrease in enamel microhardness after the application of theobromine gel and acid immersion (p <0.05). The highest values of increase and decrease in enamel microhardness (764,74±187,08 VHN and 457,18±54,37 VHN, respectively) was shown in 5% group of theobromine gel.
Conclusion: The higher theobromine gel concentration, the higher the microhardness value but unable to maintain the enamel resistance against acid. The 5% theobromine gel concentration was the most effective one to increase the microhardness of primary teeth enamel.

Keywords: Theobromine; hardness resistance; primary teeth enamel

  1. Gunawan, A.H., Pengaruh Perubahan Kristal Apatit, Tingkat Retensi Dan Intrusi Flour Terhadap Kelarutan Email Setelah Perlakuan Larutan Ikan Teri Jengki (S. insularis),Disertasi, Program Doktor Fakultas Kedokteran Gigi Universitas Indonesia, Jakarta,2006.
  2. Herisa, H.M., Noerdin, A., dan Eriwati, Y.K., The effect of theobromine 200mg/l topical gel exposure duration against surface enamel hardness resistance from 1% citric acid, Journal of Physics: Conference Series;2017: 884(012009).
  3. Alani B.W., Qasim A.A., The effect of topical fluoride products on surface microhardness of enamel of primary teeth (An in vitro study), International Journal of Enhanced Research in Science Technology & Engineering, 2014;3(9): 147-153.
  4. Lee, Mi Ra, Lee, Chung Jae, Park, Ji Hyeon, The Roughness & Micro-hardness on the Deciduous Teeth according to Formula Milk or Human Milk, International Journal of Clinical Preventive Dentistry, 2011;7(4): 179-88.
  5. McCabe, J.F. dan Walls, A.W.G., Applied Dental Materials, edisi ke-9, Blackwell Publishing, Oxford, 2008; 12 –14.
  6. Hatrick, C.D, Eakle, W.S, Bird, W.F, Dental materials: Clinical Applications for Dental Assistans and Dental Hygienists, 2nd ed.,Saunders Elsevier: USA, 2011.
  7. Sadeghpour, A, A Neural Network Analysis of Theobromine vs. Fluoride on The Enamel Surface of Human Teeth: an Experimental Case Study with Strong Implications for The Production of a New Line of Revolutionary and Natural Non-Fluoride Based Dentifrices, Dissertation Abstracts International, 2007;68 (7).
  8. Sulistianingsih, Irmaleny, Hidayat O.T, The remineralization potential of cocoa bean extract (Theobroma cacao) to increase the enamel microhardness, Padjajaran Journal of Dentistry, 2017; 29(2).
  9. Nasution, A.I, Zawil C, The comparison of enamel hardness between fluoride and theobromine application, International Journal of Contemporary Dental and Medical Reviews, 2014
  10. Haghgou H.R., Haghgoo R., Asdollah, F.M., Comparison of the microhardness of primary and permanent teeth after immersion in two types of carbonated beverages, J of International Society of Preventive and Community Dentistry, 2016;6(4): 342-47.
  11. Hamzah, M.M., Semreen M. H., Naddaf, A.R., Anti-inflammatory activity of Achillea and Ruscus topical gel on carrageenan-induced paw edema in rats, Natural Drugs, 2006;63(4): 277-300
  12. Jiang, H., Liu, X.Y., Lim, C.T., Hsu, C.Y., Ordering of self assembled nanobiominerals in correlation to mechanical properties of hard tissues, Appl Phys Lett, 2005;86:163901.
  13. Eimar H., Tooth Enamel Ultrastructure: Correlation between Composition and Physical Properties, Thesis, Faculty of Dentistry, McGill Univ., Montreal, Quebec, Canada, 2011; 21-34.
  14. Nakamoto, T., Simmons, W.B., dan Falster, A.U., Apatite-Forming-Systems: Methods and Products: United States Patent, 2001; 1 – 33.
  15. Kargul, B., Ozcan, M., Peker, S., Nakamoto, T., Simmons, W.B., Falster, A.U., Evaluation of Human Enamel Surfaces Treated with Theobromine: a Pilot Study, Oral Health & preventive dentistry, 2012; 10(3).
  16. Amaechi, B.T., Porteous, N., Ramalingam, K., Mensinkai, P.K., Vasquez R.A.C, Sadeghpour, A., dan Nakamoto, T., Remineralization of Artificial Email Lesions by Theobromine, Caries Research, 2013;47: 399–405.
  17. Kidd, E.A.M., Fejerskov, O., Dental Caries: The Disease and Its Clinical Management, Blackwell Munksgaard: Oxford, 2003;50-69.
  18. Kuroiwa, M, Acid Resistance of Surface - ePrismless' Enamel in Human Deciduous and Permanent Teeth, Showa Univ. J. Med. Sci, 1990;2(1): 31-44
  19. Oliveira, M.A.H., Torres C.P., Gomes-Silva, J.M., Chinelatti, M.A., De Menezes, F.C.H., Palma-DIbb, R.G., Borsatto, M.C., Microstructure and Mineral Composition of Dental Enamel of Permanent and Deciduous Teeth, Microscopy Research and Technique, 2010;73: 572-77.
  20. Junaidi, L., Sudibyo, A., Hendarti, Penelitian Ekstraksi Theobromine dari Biji Kakao, J.of Agro-Based Industry,2007; 24(1): 9-12.
  21. Meerbeek, V.B., Munck, D.J., Yoshida, Y.,Inoue, S., Vargas, M., Vijay, P., Van Landuyt, K., Lambrechts, P., Vanherle, G., Adhesion to enamel and dentin: current status and future challenges, Oper Dent, 2003;28(3): 215-35.
  22. Shahmoradi, M., Bertassoni, L.E., Elfallah, H.M., Swain, M., Fundamental Structure and Properties of Enamel, Dentin and Cementum dalam Advances in calcium phosphate biomaterials, Biomaterials Science and Engineering, 2014;2: 411-547.
  23. European Patent Specification, Compositions comprising theobromine for the prevention of caries, 2010, EP 2 533 786 B1.

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