Effect of Celery Extract Administration on 8-OHdG and Number of Foam Cell in Wistar Strain Rats With A High-Fat Diet

Agnes Budiarto*  -  Master of Biomedical Sciences, Faculty of Medicine Sultan Agung Islamic University, Semarang, Indonesia
Taufiqurrachman Nasihun  -  Master of Biomedical Sciences, Faculty of Medicine Sultan Agung Islamic University, Semarang, Indonesia
Atina Hussaana  -  Master of Biomedical Sciences, Faculty of Medicine Sultan Agung Islamic University, Semarang, Indonesia

(*) Corresponding Author
DOI: 10.30659/sainsmed.v8i2.2421

BACKGROUND: A high fat diet, accompanied by high frequency eating, results in hyperlipidemia. One prevention for hyperlipidemia is administration of celery extract.

OBJECTIVE: to explore the effect of celery extract on the level of 8-OHdG (8 -Hydroxy-2’-Deoxy Guanosine) and the number of foam cell.


METHODS: Experimental studies with post-test only control group design used Wistar rats, administered with adrenaline and high-fat diet for
hyperlipidemic induction.Total of 35 rats were divided into 5 groups: negative controls (Neg-G); positive control treated with simvastatin (Nor-G); ES25-G,ES50-G, and ES75-G group were treated with 25 mg/200 g BW, 50 mg/200 g BW, and 75 mg/200 g BW celery extract, respectively. The treatment was given for 30 days. The 8-OHdG level was determined by ELISA, whereas the number of foam cells was determined by histopathologic preparations with HE staining. Data analysis was conducted by Kruskal-Wallis and Mann-Whitney.


RESULTS: Mann-Whitney analysis showed levels of 8-OHdG in ES-25-G 12.13±0.21, ES-50-G 7.23±0.25, ES-75-G 4.41±0.45 significantly lower
than the Nor-G 14.30±0.66, respectively p<0.001. The number of foam cells in the ES-25-G was 7.57±0.53, ES-50-G 6.57±0.79, ES-75-G 3.43±0.53,significantly lower than the Neg-G 13.57±1.27, respectively p<0.001.

CONCLUSION: Celery extract capable of decreasing levels of 8-OHdG and the number of foam cells in Wistar rats induced with adrenaline and high-fat
diet.

Keywords: celery;8-OHdG;foam cell

  1. Anggraeni, T., Ridwan, A. & Kodariah, L., 2016. Ekstrak Etanol Seledri (Apium graveolens) Sebagai Anti-Atherogenik Pada Tikus (Rattus norvegicus) Yang Diinduksi Hiperlepidemia. Prosiding Symbion (Symposium on Biology Education), pp.171–188. Available at: http://symbion.pbio.uad.ac.id/prosiding/prosiding/ID_298_Tjandra-Liah_revisi_Hal 171-188.pdf. [Cited: Oktober 2017]
  2. Dalimartha, S., 2011. 36 Resep Tmbuhan Obat Untuk Menurunkan Kolestrol, Jakarta: Penebar Swadaya.
  3. Donald Lloyd-Jones, Robert Adams, 2009. At a Glance: Heart Disease and Stroke Prevention. ahajournals, 119, pp.e21–e181. Available at: http://circ.ahajournals.org/content/119/3/e21. [Cited: April 2017]
  4. Dwi, N.G.A.M., Suastuti, A., Sri, I.G.A.K., Dewi, P., Ni, D., et al., 2015. PEMBERIAN EKSTRAK DAUN SIRSAK (Annona muricuta) UNTUK MEMPERBAIKI KERUSAKAN SEL BETA PANKREAS MELALUI PENURUNAN KADAR GLUKOSA DARAH, ADVANCED GLYCATION AND PRODUCT DAN 8-HIDROKSI-2-DIOKSIGUANOSIN PADA TIKUS WISTAR HIPERGLIKEMIA. JURNAL KIMIA, 9(2)(JULI), pp.289–295. Available at: http://download.portalgaruda.org/article.php?article=365638&val=931&title=PEMBERIAN EKSTRAK DAUN SIRSAK (Annona muricuta) UNTUK MEMPERBAIKI KERUSAKAN SEL BETA PANKREAS MELALUI PENURUNAN KADAR GLUKOSA DARAH, ADVANCED GLYCATION AND PRODUCT DAN 8-HIDROKSI-2-. [Cited: April 2017]
  5. Fitria, 2016. Khasiat Daun Seledri (Apium graveolens) Terhadap Tekanan Darah Tinggi Pada Pasien Hiperkolestrolemia. Journal of Chemistry UNUD, 5(2), p.120. Available at: https://ccs.infospace.com/ClickHandler.ashx?encp=ld%3D20180308%26app%3D1%26c%3DAV772%26s%3DAV%26rc%3DAV772%26dc%3D%26euip%3D112.215.237.95%26pvaid%3Dbbda396ce1344fc09787956159b6b1f9%26dt%3DDesktop%26sid%3D606190505.2023246724585.1520481016%26vid%3D6061905. [Cited: April 2017]
  6. Iflahah, M.A. et al., 2016. Aktivitas Antioksidan Biji Kakao ( Theobroma cacao L .) Dalam Menurunkan Kadar 8-Hidroksi- 2 ’ -Deoksiguanosin. Indonesian E-Journal of Applied Chemistry, 4(2), pp.113–119. Available at: https://ojs.unud.ac.id/index.php/cakra/article/view/28926. [Cited: April 2017]
  7. Kohen, R., Nyska, A., 2002. Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions,and methods for their quantification. The Society of Toxicologic Pathology, 30(6), pp.620–650. Available at: https://www.ncbi.nlm.nih.gov/pubmed/12512863. [Cited: April 2017]
  8. Loho, L.L. & Lintong, P.M., 2015. Pengaruh Pemberian Ekstrak Kunyit Terhadap Gambaran Histopatologi Aorta Tikus Wistar ( rattus novergicus ) Hiperlipidemia. Journal e-Biomedik (eBM), 3(1), pp.199–204. Available at: https://media.neliti.com/media/publications/68626-ID-none.pdf. [Cited: March 2017]
  9. Maramis. R., Kaseke . M., T.G.., 2014. Gambaran Histologi Aorta Tikus Wistar Dengan Diet Lemak Babi Setelah Pemberian Ekstrak Daun Sirsak (Annona muricata). Jurnal e-Biomedik (eBM), 2(2). Available at: https://ejournal.unsrat.ac.id/index.php/ebiomedik/article/view/4702. [Cited: April 2017]
  10. Nur, S. et al., 2012. Potensi Antioksidan dalam Ekstrak Teh Merah ( Hibiscus sabdariffa ) dan Teh Hijau ( Camellia sinensis ) terhadap Proses Aterogenesis pada Tikus dengan Diet Aterogenik ( Antioxidant Potential of the Extract of Green Tea ( Camellia sinensis ) and Red Tea. Journal Unair, 14(3), pp.158–171. Available at: http://journal.unair.ac.id/download-fullpapers-Biosains Vol 14 No 3 September 2012-6.pdf. [Cited: Febuari 2017]
  11. Riskesdas, 2010. Riskesdas: Badan Penelitian dan Pengembangan Kesehatan, Kementrian Kesehatan RI.
  12. Siska., Farida, K., 2010. Pemanfaatan Akar Seledri (Apium gravelens) sebagai Anti Hipertensi. Journal of Farmasains UHAMKA, 1(1). Available at: http://farmasains.uhamka.ac.id/volume-1-nomor-1/pemanfaatan-akar-seledri-apium-graveolens-linn-sebagai-antihipertensi/. [Cited: April 2017]
  13. Susantiningsih, T., 2015. Obesitas dan Stres Oksidatif. Juke Unila, 5(9), pp.89–93. Available at: http://juke.kedokteran.unila.ac.id. [Cited: April 2018]
  14. Umarudin & Susanti, 2012. Efektifitas ekstrak tanin seledri terhadap profil lipid tikus putih hiperkolesterolemi. Unnes Journal of Life Science, 1(2), pp.78–85. Available at: http://lib.unnes.ac.id/15106/. [Cited: April 2017]
  15. Valavanidis A, Vlachogianni T, Fiotakis K, L.S., 2013. Pulmonary oxidative stress, inflammation and cancer: respirable particulate matter, fibrous dusts and ozone as major causes of lung carcinogenesis through reactive oxygen species mechanisms. Int J Environ Res Public Health, 10(9), pp.3886–907. Available at: http://www.mdpi.com/1660-4601/10/9/3886. [Cited: April 2017]

Open Access Copyright (c) 2018 Sains Medika: Jurnal Kedokteran dan Kesehatan
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Sains Medika: Jurnal Kedokteran dan Kesehatan
is published by Faculty of Medicine Universitas Islam Sultan Agung, Indonesia in association with FOKI (FORUM KEDOKTERAN ISLAM INDONESIA).

Contact: Jl. Raya Kaligawe Km.4, PO BOX 1054/SM Semarang 50112, Indonesia
Phone: +62 8122-9933-369
Website: https://fkunissula.ac.id
Email: sainsmedika@unissula.ac.id

ISSN: 2339-093X (Online) | 2085-1545 (Print)
DOI : 10.30659/sainsmed

This work is licensed under a Creative Commons Attribution 4.0 International License

Get a feed by atom here, RRS2 here and OAI Links here

apps